Basidiospore germination of Calvatia gigantea - Here they did further experiments on the role of red yeast for spore germination. Again, success on PDA and MEA only happened together with live yeast, on less complex media also without, but in very little numbers. The authors conclude that these yeasts produce stimulating substances as well as remove others which are suppressing spore germination. Wilson, R.W.; Beneke, E.S. Mycologia 1966
Studies on Calvatia gigantea. II. Factors affecting basidiospore germination - Spores germinated at 24 - 26°C after several months, only in low numbers (a few per million) and only in presence of a red yeast (Rhodotorula mucilaginosa) or an unidentified bacterium. Live mycelium of a giant puffball did not stimulate spore germination. Usable additions to agar were barley extract, malt extract and an extract from giant puffballs. Bulmer, G.S.; Beneke, E.S. Mycologia 1962
Physiological Studies on Calvatia Species. II. Carbon Utilization - Four puffball strains (3x C. gigantea, 1x C. fragilis) had been tested in Lindbergs medium with thiamine (100µg/l) and 24 different carbohydrates. They found that the giant puffball apparently could not utilize cellulose, while starch was of little use. Their decomposition products cellobiose and dextrin worked very well, even better than glucose or maltose. Regarding the inability to use cellulose, they mention the possibility of faulty results, as their cellulose wasn't soluble. As their medium was constantly shaken, this warning can possibly be ignored. M. Sedlmayr, E.S. Beneke, J.A. Stevens, Mycologia 1961
Physiological Studies on Calvatia Species I. Vitamin Requirements - Fours strains of the giant puffball had been tested in a vitamin free liquid medium (Lindbergs medium, 2% Glucose and salts) at PH 5.5 and 25°C with various supplements. They found that the mycelium required thiamine (Vitamin B1, min. 25µg/l) in the medium and that bacterial peptone (0.5%) and yeast extract (0.5%) dramatically accelerated mycelium growth. M. Sedlmayr, E.S. Beneke, J.A. Stevens, Mycologia 1961
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